Mobile Suit Gundam: Iron-Blooded Orphans: Squeeze

Biscuit Griffon being hugged by Cookie Griffon and Cracker Griffon at the same time

Biscuit Griffon works for a private security company (and then a mercenary group) in order to make enough money to support his family and send Cookie and Cracker, his younger twin sisters, to school.
From the anime series Mobile Suit Gundam: Iron-Blooded Orphans

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BioShock Infinite: It’s Complicated

Robert Lutece stands in the dark, holding a coin with his thumb and forefinger. Rosalind Lutece stands next to him.

Twins Rosalind Lutece and Robert Lutece are both physicists who are, had been, and will be one of a kind.
From the video game BioShock Infinite

The Loud House: Turn Up the Volume

The Loud siblings (Lori, Leni, Luna, Luan, Lynn, Lincoln, Lucy, Lana, Lola, Lisa, and Lily) gathered on and around a couch watching television

There are eleven children in the Loud household: Lori the eldest is always on her phone; Leni the second eldest is fashionable and ditzy; Luna is a rock musician; Luan is an aspiring comedian; Lynn is an athlete who loves sports; Lincoln the middle child is the only boy; Lucy is a pale-skinned goth; Lana is a tomboy who enjoys building and fixing things; her twin sister, Lola, loves wearing pink dresses; Lisa is a child prodigy, and Lily is a baby.
From the Nickelodeon series The Loud House

Baka to Test to Shoukanjuu: Tea for Two

Yuuko Kinoshita and Hideyoshi Kinoshita seated and drinking from a cup

Yuuko Kinoshita and her younger twin brother, Hideyoshi, might look identical, but they’re quite different. She’s an intelligent, prideful, and irritable member of Fumizuki Academy’s top class, whereas he’s a helpful and kind individual whose true passion is acting.
From the anime series Baka to Test to Shoukanjuu

Bubuki Buranki: Rivals

Close-up of Asuma Kazuki and Kaoruko angrily butting their heads together

Buranki are giant, sentient, robotic beings, and both Azuma Kazuki and his younger twin sister, Kaoruko, have the power to awaken and use the one known as Oubo, though they disagree over who has the right to possess its control-unit “heart.”
From the anime series Bubuki Buranki

Gakusen Toshi Asterisk: Students of the Yellow Dragon

Shenhua Li and Shenyun Li wearing pink fighting clothing

Shenyun Li and his younger twin sister, Shenhua, are both arrogant and ruthless but highly skilled fighters who enjoy making their opponents suffer in the arena.
From the anime series Gakusen Toshi Asterisk (The Asterisk War: The Academy City on the Water)

Amanchu!: Merriment

Makoto Ninomiya and Ai Ninomiya wearing wetsuits in front of a polka dot background

When Ai Ninomiya is not busy kicking her younger twin brother, Makoto, for fun, the two enjoy going scuba diving.
From the anime series Amanchu!