Shoutarou Torii and his little sister, Moa, do not get along. He finds her rude and mean most of the time, especially when she calls him “baldy”, while she considers him to be loud, annoying, and shamelessly perverted. That said, he won’t hesitate to protect her if she’s in trouble.
From the anime series Saikin, Imouto no Yousu ga Chotto Okashiinda ga. (Recently, My Sister is Unusual)
Rich kid Miku Sugisaki is haughty and loves to brag about her expensive clothes and possessions, while her younger brother, Ryuuta, is ill-mannered and selfish. That said, she does try to make sure he stays away from bad influences.
From the anime series Mitsudomoe
Sorata Kanda lives in a co-ed dormitory with many pretty girls, much to the dismay of his possessive and adoring little sister, Yuuko.
From the anime series Sakurasou no Pet na Kanojo (The Pet Girl of Sakurasou)
Akuru Akutsu loves doing two things: doting on his little sister, Ruri, and studying for school.
From the anime series Aho-Girl
Hazuki Yazakura has a different last name than her younger brother, Chitose Hitotose, and younger sister, Minazuki Rokumatsuri, because they’re orphans who became self-adopted siblings.
From the anime series Happy Lesson
Image from the anime series Happy Lesson
Kirino Kosaka forces her older brother, Kyosuke, to give her life counseling advice about how she can be more open about her hobbies. It also serves as a way to strengthen their bonds as siblings after years of neglect.
From the anime series Ore no Imouto ga Konnani Kawaii Wake ga Nai. (My Little Sister Can’t Be This Cute.)
Chris Yamada is the executive editor for his little sister, the light novel author known publicly as Elf Yamada. He has to keep an eye on her to ensure she’s actually working because she’s known to be lazy and miss her deadlines.
From the anime series Eromanga Sensei
With their father always working and their mother having passed away, Kasumi Kagami assumed the responsibility of taking care of her younger brother, Kazuya. She can be a bit obsessive when it comes to his safety and well-being, though. (And from his perspective, the less said about her desire to tend to his physical needs, the better.)
From the anime series Tsugumomo
Rockman (Mega Man) and Roll are sibling robots who were created by Dr. Light to be his assistants, but now they work to defeat the rogue robotic creations of Dr. Wily.
From the anime series Rockman Hoshi ni Negai wo (Mega Man: Wish Upon a Star)